Purification of the pro-phenol oxidase enzyme from haemocytes of the cockroach Blaberus discoidalis.
نویسندگان
چکیده
Pro-phenol oxidase was purified from the haemocytes of the cockroach Blaberus discoidalis by Blue Sepharose chromatography, hydrophobic-interaction chromatography on a Phenyl-Superose column and, finally, gel filtration on a Superose 6 column. Results suggest that the molecule exists as a polymer of identical 76 kDa monomeric units. The enzyme is a glycoprotein with pI of 5.2 and can be converted by trypsin into phenol oxidase.
منابع مشابه
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عنوان ژورنال:
- The Biochemical journal
دوره 289 ( Pt 1) شماره
صفحات -
تاریخ انتشار 1993